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991.
Anna Capaldo Flaminia Gay Massimo Maddaloni Salvatore Valiante Maria De Falco Mauro Lenzi Vincenza Laforgia 《Water, air, and soil pollution》2012,223(5):2137-2143
The presence of illicit drugs and their metabolites in surface waters has to be considered a new type of hazard, still unknown, for the aquatic ecosystem, due to the potent pharmacological activities of all the illicit drugs. Our research was therefore aimed at evaluating the impact of illicit drugs on the aquatic fauna, till now still undervalued. To this aim, we verified the ability of the European eel (Anguilla anguilla), a well-known biomonitor of environmental contamination, to bioaccumulate cocaine, one of the most abundant illicit drugs found in surface waters. Silver eels were exposed to a nominal cocaine concentration of 20?ng/l for 1?month; at the same time, control, carrier, and post-exposure recovery groups were made. Brains, gills, liver, kidney, muscle, gonads, spleen, digestive tract, and sections of dorsal skin were assayed by high-pressure liquid chromatography. Cocaine was found in the tissues of the treated eels and, at low concentrations, in almost all tissues of post-exposure recovery eels. These results indicate that cocaine is able to accumulate into the eel tissues; its presence suggests potential risks for eels since cocaine could affect their physiology and contribute to their decline, and for humans consuming contaminated fish. 相似文献
992.
Olivera Stajkovi?-Srbinovi? Sofie E. De Meyer Bogi? Mili?i? Du?ica Deli? Anne Willems 《Biology and Fertility of Soils》2012,48(5):531-545
We have evaluated the genetic diversity and phylogeny of alfalfa rhizobia, originating from different types of soils in Serbia and their ability to establish an effective symbiosis with alfalfa (Medicago sativa L.). A collection of 65 strains isolated from root nodules of alfalfa were characterized by rep-PCR analysis, partial and complete 16S rDNA gene and recA gene sequencing, as well as atpD gene sequencing and DNA–DNA hybridizations. The results of the sequence analyses revealed that Sinorhizobium meliloti is the dominant species in alfalfa nodules. Only one strain was identified as Sinorhizobium medicae, two strains as Rhizobium tibeticum and one strain as Rhizobium sp. Despite the fact that the majority of strains were identified as S. meliloti, a high genetic diversity at strain level was detected. Almost all isolates shared the ability to nodulate and fix nitrogen with M. sativa, except 11 of them, which were incapable of fixing nitrogen with this species. About 50% of the isolates showed values of symbiotic effectiveness (SE) above 50%, while 10% of the strains were highly effective with SE values above 70%. Some of the strains which were highly effective in nitrogen fixation at the same time could intensively solubilize phosphates, offering a possibility for multipurpose inoculum development. This was the first genetic study of rhizobia isolated from this region and also the first report of natural presence of R. tibeticum in root nodules of M. sativa. 相似文献
993.
Federico Rossi Ruth M. Potrafka Ferran Garcia Pichel Roberto De Philippis 《Soil biology & biochemistry》2012
Biological soil crusts (BSCs) are highly specialized topsoil microbial communities commonly found in arid and semiarid environments, permeated by a polymeric matrix of polysaccharides. BSCs can in principle influence edaphic properties such as texture, pore formation and water retention, which in turn determine water distribution and biological activity in dry lands. This paper investigates the influence of biotic and abiotic factors on BSC hydraulic conductivity, a parameter gauging the ease with which water can move through the pore spaces. Texture, phototroph abundance, microbial composition, and extracellular carbohydrate content were considered as potentially relevant parameters in a correlational study of BSC samples that spanned 1.5 orders of magnitude in hydraulic conductivity. A newly developed, non-destructive extraction method enabled us to directly quantify the specific role of extracellular polysaccharides on soil permeability on a variety of samples. Hydraulic conductivity showed a strongest correlation with texture (positive with sand content, negative with silt and clay). A weaker negative correlation with carbohydrate content, especially with polysaccharides having a molecular weight < 100 kDa, was also detected. In multiple regression analyses texture (silt content) was sufficient to explain most of the variation in hydraulic conductivity However, experimental removal of polymeric carbohydrates, resulted invariably in a substantial decrease in hydraulic conductivity for any given sample (between 1.7 and 3.3 fold). Our results suggest that while soil texture determines overall hydraulic conductivity in BSCs, the presence of exopolysaccharides can significantly enhance it, likely by conferring a spongy structure to a BSC thus increasing the number of waterways within it. 相似文献
994.
D Martínez-Maqueda B Miralles S De Pascual-Teresa I Reverón R Muñoz I Recio 《Journal of agricultural and food chemistry》2012,60(35):8600-8605
In this study, the hypothesis that food-derived opioid peptides besides β-casomorphin 7 might modulate the production of mucin via a direct action on epithelial goblet cells was investigated in HT29-MTX cells used as a model of human colonic epithelium. Seven milk whey or casein peptides, a human milk peptide, and a wheat gluten-derived peptide with proved or probable ability to bind μ- or δ-opioid receptors were tested on the cell culture. Significantly increased secretion of mucins was found after exposure to six of the assayed peptides, besides the previously described β-casomorphin 7, as measured by an enzyme-linked lectin assay (ELLA). Human β-casomorphin 5 and α-lactorphin were selected to study the expression of mucin 5AC gene (MUC5AC), the HT29-MTX major secreted mucin gene. α-Lactorphin showed increased expression of MUC5AC from 4 to 24 h (up to 1.6-fold over basal level expression), although differences were statistically different only after 24 h of exposure. However, this increased expression of MUC5AC did not reach significance after cell treatment with human β-casomorphin 5. In conclusion, six food-derived peptides have been identifed with described or probable opioid activity that induce mucin secretion in HT29-MTX cells. Concretely, α-lactorphin is able to up-regulate the expression of the major secreted mucin gene encoded by these cells. 相似文献
995.
S. Landschoot W. Waegeman K. Audenaert G. Haesaert B. De Baets 《Plant pathology》2013,62(6):1319-1329
Deoxynivalenol (DON) is one of the most prevalent toxins in Fusarium‐infected wheat samples. Accurate forecasting systems that predict the presence of DON are useful to underpin decision making on the application of fungicides, to identify fields under risk, and to help minimize the risk of food and feed contamination with DON. To this end, existing forecasting systems often adopt statistical regression models, in which attempts are made to predict DON values as a continuous variable. In contrast, this paper advocates the use of ordinal regression models for the prediction of DON values, by defining thresholds for converting continuous DON values into a fixed number of well‐chosen risk classes. Objective criteria for selecting these thresholds in a meaningful way are proposed. The resulting approach was evaluated on a sizeable field experiment in Belgium, for which measurements of DON values and various types of predictor variables were collected at 18 locations during 2002–2011. The results demonstrate that modelling and evaluating DON values on an ordinal scale leads to a more accurate and more easily interpreted predictive performance. Compared to traditional regression models, an improvement could be observed for support vector ordinal regression models and proportional odds models. 相似文献
996.
997.
Susana Guill��n Teresa Terrazas Erick De la Barrera Alejandro Casas 《Genetic Resources and Crop Evolution》2011,58(3):409-423
Germination of wild and managed populations of columnar cacti was compared to analyze differences associated to management intensity. The species studied: Polaskia chende, Escontria chiotilla, Myrtillocactus schenckii, Polaskia chichipe, and Stenocereus pruinosus are in a gradient from lower to higher management intensity, respectively. Within each species wild, silvicultural, and cultivated populations also represent a gradient from lower to higher management intensity. We hypothesized that seeds of plants under higher management intensity would require more water to germinate than others since managed environments are relatively wetter than unmanaged environments. Such pattern could be visualized within and among species according to management intensity. We tested germination of seeds from different populations of the different species to the water potentials: 0.0, ?0.2, ?0.4, ?0.6, ?0.8, and ?1.0 MPa. In all species studied seeds of managed populations had significantly more mass than seeds from wild populations. Each species had different susceptibility to water availability decrease, which could be related to particular adaptations to drought resistance. Stenocereus pruinosus and Polaskia chende were the most and least susceptible species, respectively. In control treatments of all species, seeds of managed populations had higher germination rate than seeds from wild populations, but when water availability decreased the pattern was the contrary. Our results suggest that artificial selection for larger fruits and heavier seeds in plants established in wetter environments have affected germination patterns. Although species have particular critical points of water availability for germination, differences in germination patterns are more pronounced according to management and artificial selection intensity. 相似文献
998.
Francesco Carimi Francesco Mercati Roberto De Michele Maria Carola Fiore Paolo Riccardi Francesco Sunseri 《Genetic Resources and Crop Evolution》2011,58(7):967-975
The Sicilian grape cultivar ‘Nero d’Avola’ is among the oldest and most cultivated in the island, taking part in the production
of several red wines exported worldwide, including DOC wines (Etna Rosso and Cerasuolo di Vittoria). Due to the ancient origin
and repeated clonally propagation of the cultivar, phenotypic variability has been observed. Clone identification in this
important cultivar has so far relied on phenotypic and chemical traits analyses, often affected by environmental conditions.
Genetic markers, such as microsatellites, are particularly useful for cultivar identification, parentage testing, pedigree
reconstruction and population structure studies. In the present paper, microsatellites were used to analyze the intra-varietal
genetic diversity among 118 plants of ‘Nero d’Avola’, collected in 30 vineyards displaced in different areas of Sicily. Out
of 22 microsatellites, 11 showed polymorphism among samples and 15 different phylogenetic groups were identified. Results
show that ‘Nero d’Avola’ actually comprises different genetic profiles, although most of clones share a common origin. 相似文献
999.
1000.
Roberta Bettinetti Silvana Galassi Silvia Quadroni Pietro Volta Fabrizio Capoccioni Eleonora Ciccotti Giulio A. De Leo 《Water, air, and soil pollution》2011,217(1-4):321-331
One of the aims of the Water Framework Directive 2000/60/CE is to assess the ecological status of water bodies in Europe in relation to priority contaminants, including some persistent organic pollutants (POPs). Recognizing the benefits of measuring hydrophobic compounds in biota tissues rather than in water, we used the European eel Anguilla anguilla in the present study as a bioindicator species for monitoring POPs in freshwater/brackish environments. We present the results of a contamination analysis of eels carried out in three Italian sites representative of different pollution levels: the last part of Tevere River, which flows through a very urbanized and industrialized area, Caprolace Lake and Lesina Lagoon, inside two different protected national parks. A very high pollution variability was recorded within each group of eels, in particular for those caught at Caprolace Lake. Due to this high variability, inter-population comparisons were carried out only between eels collected in Tevere River and Lesina Lagoon. PCBs values in Lesina Lagoon were two orders of magnitude lower than those measured in Tevere River, while no significant differences were found for levels of DDT compounds. Our results confirm the suitability of the eel as an indicator of persistent bioaccumulative pollutants in brackish environments and rivers where it can be easily caught, but we are sceptical of its use in deep lakes where its capture might be problematic considering standard operation procedures for fish sampling. 相似文献